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rabbit anti sirtuin1  (Proteintech)


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    Structured Review

    Proteintech rabbit anti sirtuin1
    IR-61 increased Nrf2 activation and restored <t>SIRT1</t> and SIRT3 expression in cavernous tissue. (a) Representative images of SIRT1 costaining with α-SMA. Scale bar = 100 μm. (b) Representative images of SIRT3 co-staining with α-SMA and quantitative analysis of the fluorescence intensity of SIRT3. Scale bar = 100 μm ( n = 4 per group). (c) Representative images of Western blot analysis of SIRT1 and SIRT3. (d) Quantitative analysis of the SIRT1 fluorescence intensity ( n = 4 per group). (e) Relative SIRT1 and SIRT3 protein levels in the cavernous tissue ( n = 4–5 each group). (f) Western blot analysis of Nrf2 and HO-1 in the cavernous tissue. (g) Relative protein levels of Nrf2 and HO-1 in the corpus cavernosum ( n = 4 per group). * P < 0.05, the indicated group compared with the control group; ** P < 0.01, the indicated group compared with the control group; *** P < 0.001, the indicated group compared with the control group; # P < 0.05, the indicated group compared with the DM group; ## P < 0.01, the indicated group compared with the DM group; ### P < 0.001, the indicated group compared with the DM group. Nrf2: nuclear factor (erythroid-derived 2)-like 2; SIRT1: <t>sirtuin1;</t> SIRT3: sirtuin3; HO-1: heme oxygenase; α-SMA: α-smooth muscle actin; DM: diabetes mellitus; DAPI: 4',6-diamidino-2-phenylindole.
    Rabbit Anti Sirtuin1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 311 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+sirtuin1/SIRT1+Polyclonal+antibody/pmc08152422-72-69-73
    Average 96 stars, based on 311 article reviews
    rabbit anti sirtuin1 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "The near-infrared dye IR-61 restores erectile function in a streptozotocin-induced diabetes modelvia mitochondrial protection"

    Article Title: The near-infrared dye IR-61 restores erectile function in a streptozotocin-induced diabetes modelvia mitochondrial protection

    Journal: Asian Journal of Andrology

    doi: 10.4103/aja.aja_69_20

    IR-61 increased Nrf2 activation and restored SIRT1 and SIRT3 expression in cavernous tissue. (a) Representative images of SIRT1 costaining with α-SMA. Scale bar = 100 μm. (b) Representative images of SIRT3 co-staining with α-SMA and quantitative analysis of the fluorescence intensity of SIRT3. Scale bar = 100 μm ( n = 4 per group). (c) Representative images of Western blot analysis of SIRT1 and SIRT3. (d) Quantitative analysis of the SIRT1 fluorescence intensity ( n = 4 per group). (e) Relative SIRT1 and SIRT3 protein levels in the cavernous tissue ( n = 4–5 each group). (f) Western blot analysis of Nrf2 and HO-1 in the cavernous tissue. (g) Relative protein levels of Nrf2 and HO-1 in the corpus cavernosum ( n = 4 per group). * P < 0.05, the indicated group compared with the control group; ** P < 0.01, the indicated group compared with the control group; *** P < 0.001, the indicated group compared with the control group; # P < 0.05, the indicated group compared with the DM group; ## P < 0.01, the indicated group compared with the DM group; ### P < 0.001, the indicated group compared with the DM group. Nrf2: nuclear factor (erythroid-derived 2)-like 2; SIRT1: sirtuin1; SIRT3: sirtuin3; HO-1: heme oxygenase; α-SMA: α-smooth muscle actin; DM: diabetes mellitus; DAPI: 4',6-diamidino-2-phenylindole.
    Figure Legend Snippet: IR-61 increased Nrf2 activation and restored SIRT1 and SIRT3 expression in cavernous tissue. (a) Representative images of SIRT1 costaining with α-SMA. Scale bar = 100 μm. (b) Representative images of SIRT3 co-staining with α-SMA and quantitative analysis of the fluorescence intensity of SIRT3. Scale bar = 100 μm ( n = 4 per group). (c) Representative images of Western blot analysis of SIRT1 and SIRT3. (d) Quantitative analysis of the SIRT1 fluorescence intensity ( n = 4 per group). (e) Relative SIRT1 and SIRT3 protein levels in the cavernous tissue ( n = 4–5 each group). (f) Western blot analysis of Nrf2 and HO-1 in the cavernous tissue. (g) Relative protein levels of Nrf2 and HO-1 in the corpus cavernosum ( n = 4 per group). * P < 0.05, the indicated group compared with the control group; ** P < 0.01, the indicated group compared with the control group; *** P < 0.001, the indicated group compared with the control group; # P < 0.05, the indicated group compared with the DM group; ## P < 0.01, the indicated group compared with the DM group; ### P < 0.001, the indicated group compared with the DM group. Nrf2: nuclear factor (erythroid-derived 2)-like 2; SIRT1: sirtuin1; SIRT3: sirtuin3; HO-1: heme oxygenase; α-SMA: α-smooth muscle actin; DM: diabetes mellitus; DAPI: 4',6-diamidino-2-phenylindole.

    Techniques Used: Activation Assay, Expressing, Staining, Fluorescence, Western Blot, Control, Derivative Assay

    Related Articles

    Immunohistochemical staining:

    Article Title: The near-infrared dye IR-61 restores erectile function in a streptozotocin-induced diabetes modelvia mitochondrial protection
    Article Snippet: TUNEL was costained with goat anti-α-SMA (1:200; ab21027, Abcam) to evaluate the apoptotic levels of CCSMCs. .. For immunohistochemical and immunofluorescence staining, the primary antibodies were rabbit anti-Desmin (1:200; Abcam, ab32362), goat anti-α-SMA (1:200; Abcam, ab21027), mouse anti-Calponin (1:200; Proteintech, 13938-1-AP, Rosemont, IL, USA), mouse anti-SM22α (1:100; Proteintech, 60213-1-Ig), rabbit anti-OPN (1:200; Abcam, ab8448), rabbit anti-Ras homolog gene family, member A (RhoA; 1:100; Proteintech, 10749-1-AP), rabbit anti-Rho-associated, coiled-coil containing protein kinase 1 (ROCK1; 1:100; Proteintech, 21850-1-AP), mouse anti-vimentin (1:200; Abcam, ab20346), rabbit anti-cleaved-caspase-3 (1:100; Abcam, ab13847), rabbit anti-sirtuin1 (SIRT1; 1:200; Proteintech, 13161-1-AP), rabbit anti-sirtuin3 (SIRT3; 1:200; Proteintech, 10099-1-AP), goat anti-NLRP3 (1:200; Abcam, ab4207), rabbit anti-caspase-1 (1:100; Abcam, ab179515), and mouse anti-apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC; 1:100; Santa Cruz Biotechnology, sc-514414, Santa Cruz, CA, USA). ..

    Immunofluorescence:

    Article Title: The near-infrared dye IR-61 restores erectile function in a streptozotocin-induced diabetes modelvia mitochondrial protection
    Article Snippet: TUNEL was costained with goat anti-α-SMA (1:200; ab21027, Abcam) to evaluate the apoptotic levels of CCSMCs. .. For immunohistochemical and immunofluorescence staining, the primary antibodies were rabbit anti-Desmin (1:200; Abcam, ab32362), goat anti-α-SMA (1:200; Abcam, ab21027), mouse anti-Calponin (1:200; Proteintech, 13938-1-AP, Rosemont, IL, USA), mouse anti-SM22α (1:100; Proteintech, 60213-1-Ig), rabbit anti-OPN (1:200; Abcam, ab8448), rabbit anti-Ras homolog gene family, member A (RhoA; 1:100; Proteintech, 10749-1-AP), rabbit anti-Rho-associated, coiled-coil containing protein kinase 1 (ROCK1; 1:100; Proteintech, 21850-1-AP), mouse anti-vimentin (1:200; Abcam, ab20346), rabbit anti-cleaved-caspase-3 (1:100; Abcam, ab13847), rabbit anti-sirtuin1 (SIRT1; 1:200; Proteintech, 13161-1-AP), rabbit anti-sirtuin3 (SIRT3; 1:200; Proteintech, 10099-1-AP), goat anti-NLRP3 (1:200; Abcam, ab4207), rabbit anti-caspase-1 (1:100; Abcam, ab179515), and mouse anti-apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC; 1:100; Santa Cruz Biotechnology, sc-514414, Santa Cruz, CA, USA). ..

    Staining:

    Article Title: The near-infrared dye IR-61 restores erectile function in a streptozotocin-induced diabetes modelvia mitochondrial protection
    Article Snippet: TUNEL was costained with goat anti-α-SMA (1:200; ab21027, Abcam) to evaluate the apoptotic levels of CCSMCs. .. For immunohistochemical and immunofluorescence staining, the primary antibodies were rabbit anti-Desmin (1:200; Abcam, ab32362), goat anti-α-SMA (1:200; Abcam, ab21027), mouse anti-Calponin (1:200; Proteintech, 13938-1-AP, Rosemont, IL, USA), mouse anti-SM22α (1:100; Proteintech, 60213-1-Ig), rabbit anti-OPN (1:200; Abcam, ab8448), rabbit anti-Ras homolog gene family, member A (RhoA; 1:100; Proteintech, 10749-1-AP), rabbit anti-Rho-associated, coiled-coil containing protein kinase 1 (ROCK1; 1:100; Proteintech, 21850-1-AP), mouse anti-vimentin (1:200; Abcam, ab20346), rabbit anti-cleaved-caspase-3 (1:100; Abcam, ab13847), rabbit anti-sirtuin1 (SIRT1; 1:200; Proteintech, 13161-1-AP), rabbit anti-sirtuin3 (SIRT3; 1:200; Proteintech, 10099-1-AP), goat anti-NLRP3 (1:200; Abcam, ab4207), rabbit anti-caspase-1 (1:100; Abcam, ab179515), and mouse anti-apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC; 1:100; Santa Cruz Biotechnology, sc-514414, Santa Cruz, CA, USA). ..



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    IR-61 increased Nrf2 activation and restored <t>SIRT1</t> and SIRT3 expression in cavernous tissue. (a) Representative images of SIRT1 costaining with α-SMA. Scale bar = 100 μm. (b) Representative images of SIRT3 co-staining with α-SMA and quantitative analysis of the fluorescence intensity of SIRT3. Scale bar = 100 μm ( n = 4 per group). (c) Representative images of Western blot analysis of SIRT1 and SIRT3. (d) Quantitative analysis of the SIRT1 fluorescence intensity ( n = 4 per group). (e) Relative SIRT1 and SIRT3 protein levels in the cavernous tissue ( n = 4–5 each group). (f) Western blot analysis of Nrf2 and HO-1 in the cavernous tissue. (g) Relative protein levels of Nrf2 and HO-1 in the corpus cavernosum ( n = 4 per group). * P < 0.05, the indicated group compared with the control group; ** P < 0.01, the indicated group compared with the control group; *** P < 0.001, the indicated group compared with the control group; # P < 0.05, the indicated group compared with the DM group; ## P < 0.01, the indicated group compared with the DM group; ### P < 0.001, the indicated group compared with the DM group. Nrf2: nuclear factor (erythroid-derived 2)-like 2; SIRT1: <t>sirtuin1;</t> SIRT3: sirtuin3; HO-1: heme oxygenase; α-SMA: α-smooth muscle actin; DM: diabetes mellitus; DAPI: 4',6-diamidino-2-phenylindole.
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    Image Search Results


    IR-61 increased Nrf2 activation and restored SIRT1 and SIRT3 expression in cavernous tissue. (a) Representative images of SIRT1 costaining with α-SMA. Scale bar = 100 μm. (b) Representative images of SIRT3 co-staining with α-SMA and quantitative analysis of the fluorescence intensity of SIRT3. Scale bar = 100 μm ( n = 4 per group). (c) Representative images of Western blot analysis of SIRT1 and SIRT3. (d) Quantitative analysis of the SIRT1 fluorescence intensity ( n = 4 per group). (e) Relative SIRT1 and SIRT3 protein levels in the cavernous tissue ( n = 4–5 each group). (f) Western blot analysis of Nrf2 and HO-1 in the cavernous tissue. (g) Relative protein levels of Nrf2 and HO-1 in the corpus cavernosum ( n = 4 per group). * P < 0.05, the indicated group compared with the control group; ** P < 0.01, the indicated group compared with the control group; *** P < 0.001, the indicated group compared with the control group; # P < 0.05, the indicated group compared with the DM group; ## P < 0.01, the indicated group compared with the DM group; ### P < 0.001, the indicated group compared with the DM group. Nrf2: nuclear factor (erythroid-derived 2)-like 2; SIRT1: sirtuin1; SIRT3: sirtuin3; HO-1: heme oxygenase; α-SMA: α-smooth muscle actin; DM: diabetes mellitus; DAPI: 4',6-diamidino-2-phenylindole.

    Journal: Asian Journal of Andrology

    Article Title: The near-infrared dye IR-61 restores erectile function in a streptozotocin-induced diabetes modelvia mitochondrial protection

    doi: 10.4103/aja.aja_69_20

    Figure Lengend Snippet: IR-61 increased Nrf2 activation and restored SIRT1 and SIRT3 expression in cavernous tissue. (a) Representative images of SIRT1 costaining with α-SMA. Scale bar = 100 μm. (b) Representative images of SIRT3 co-staining with α-SMA and quantitative analysis of the fluorescence intensity of SIRT3. Scale bar = 100 μm ( n = 4 per group). (c) Representative images of Western blot analysis of SIRT1 and SIRT3. (d) Quantitative analysis of the SIRT1 fluorescence intensity ( n = 4 per group). (e) Relative SIRT1 and SIRT3 protein levels in the cavernous tissue ( n = 4–5 each group). (f) Western blot analysis of Nrf2 and HO-1 in the cavernous tissue. (g) Relative protein levels of Nrf2 and HO-1 in the corpus cavernosum ( n = 4 per group). * P < 0.05, the indicated group compared with the control group; ** P < 0.01, the indicated group compared with the control group; *** P < 0.001, the indicated group compared with the control group; # P < 0.05, the indicated group compared with the DM group; ## P < 0.01, the indicated group compared with the DM group; ### P < 0.001, the indicated group compared with the DM group. Nrf2: nuclear factor (erythroid-derived 2)-like 2; SIRT1: sirtuin1; SIRT3: sirtuin3; HO-1: heme oxygenase; α-SMA: α-smooth muscle actin; DM: diabetes mellitus; DAPI: 4',6-diamidino-2-phenylindole.

    Article Snippet: For immunohistochemical and immunofluorescence staining, the primary antibodies were rabbit anti-Desmin (1:200; Abcam, ab32362), goat anti-α-SMA (1:200; Abcam, ab21027), mouse anti-Calponin (1:200; Proteintech, 13938-1-AP, Rosemont, IL, USA), mouse anti-SM22α (1:100; Proteintech, 60213-1-Ig), rabbit anti-OPN (1:200; Abcam, ab8448), rabbit anti-Ras homolog gene family, member A (RhoA; 1:100; Proteintech, 10749-1-AP), rabbit anti-Rho-associated, coiled-coil containing protein kinase 1 (ROCK1; 1:100; Proteintech, 21850-1-AP), mouse anti-vimentin (1:200; Abcam, ab20346), rabbit anti-cleaved-caspase-3 (1:100; Abcam, ab13847), rabbit anti-sirtuin1 (SIRT1; 1:200; Proteintech, 13161-1-AP), rabbit anti-sirtuin3 (SIRT3; 1:200; Proteintech, 10099-1-AP), goat anti-NLRP3 (1:200; Abcam, ab4207), rabbit anti-caspase-1 (1:100; Abcam, ab179515), and mouse anti-apoptosis-associated speck-like protein containing a caspase-recruitment domain (ASC; 1:100; Santa Cruz Biotechnology, sc-514414, Santa Cruz, CA, USA).

    Techniques: Activation Assay, Expressing, Staining, Fluorescence, Western Blot, Control, Derivative Assay